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17
Connection of N
2
(or O
2
) gas cylinder
WARNING
Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are
connected correctly and are not liable to become disconnected. Ensure that the gas pressure is
set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure
may result in leakage of gas. Elevated level of gas can be hazardous to health and may lead to
asphyxiation and risk of death.
This incubator needs N
2
or O
2
gas depending on the setting of O
2
density. The selection is as follows:
When the setting of O
2
density is less than 18%: N
2
gas
When the setting of O
2
density is more than 22%: O
2
gas
O
2
density in the atmosphere is about 20%. For the control of O
2
density in the chamber, O
2
gas is
diluted by N
2
gas when the setting of O
2
density is less than that of the atmosphere. On the contrary, O
2
gas is added when the setting of O
2
density is more than that of the atmosphere.
1. Install a gas pressure regulator (optional accessory MCO-100L) on N
2
or O
2
gas cylinder. Or use a
regulator rated at 25 MPaG (250 kgf/cm
2
G, 3600 psiG) on the primary side and 0.2 MPaG (2.0 kgf/cm
2
G,
30 psiG) on the secondary side.
2. Using the gas tube provided, connect the pressure regulator to the connecting port A/B for N
2
/O
2
gas
pipe located at the rear left side of the incubator. Connect the main cylinder to the connecting port A for
N
2
/O
2
gas pipe and connect the sub-cylinder to the connecting port B for N
2
/O
2
gas pipe so that the gas
supply can be switched automatically when one gas cylinder is empty. Connect the same gas type to
both connecting port A/B for N
2
/O
2
gas pipe. Never connect N
2
gas and O
2
gas at the same time to the
connecting port A/B for N
2
/O
2
gas pipe respectively.
3. Set the N
2
or O
2
gas pressure on the secondary side to 0.05 MPaG (0.5 kgf/cm
2
G, 7.1 psiG) (at gas
injection). The higher gas pressure makes control range of O
2
density wider. In addition, excessive
pressure may cause disconnection of internal pipes inside the incubator, which will result in leakage of N
2
or O
2
gas into the atmosphere. Elevated level of CO
2
gas or O
2
gas can be hazardous to health and
may lead to asphyxiation and risk of death or fire by gas leakage. The repair of the incubator will be
necessary if the internal pipe is disconnected.
4. Check that no gas is leaking at any point where the pipe connects with the gas regulator or the
incubator.
z
The gas lines connected to the Incubator will degrade over time. If any deterioration or abnormalities
are found during inspection, replace the lines immediately.
WARNING
O
2
gas increases the susceptibility of substances to burn. Take care of the handling of flame in a room
where the incubator is installed.